| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196304 |
| Chromosome: | chromosome 12 |
| Location: | 7153472 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558950 | (1 of 1) IPR000104//IPR009071 - Antifreeze protein, type I // High mobility group box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATAAGGCATCGCCTTCTACTTAAACACG |
| Internal bar code: | CTGTGCGCCATCCCAACTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 99.3447 |
| LEAP-Seq n confirming: | 2274 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACTTTCACTGCCCGCTCT |
| Suggested primer 2: | GATCATCATGTCGTTGTGGC |