Insertion junction: LMJ.RY0402.196319_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.196319
Chromosome:chromosome 8
Location:2522265
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGTTCTCGAACCGTCGCGGCCCTTCGCG
Internal bar code:GCATTCACGCCGATCTCTAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:538
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCAGACCAGACCAGACC
Suggested primer 2:AGCGGTCTCCTTCATCTTGA