| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196358 |
| Chromosome: | chromosome 17 |
| Location: | 1111802 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g704000 | (1 of 2) 1.1.3.30 - Polyvinyl-alcohol oxidase / PVA oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGGTTAATGGTTGTACGCCGTTACGC |
| Internal bar code: | GTGGTTCGACAGGTAGCAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.8082 |
| LEAP-Seq n confirming: | 3123 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTTATGCGTTCTCATGC |
| Suggested primer 2: | CAGGAGAAGGATTACGAGCG |