| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196371 |
| Chromosome: | chromosome 13 |
| Location: | 5099424 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607350 | CAH7 | (1 of 2) PTHR11002:SF13 - BETA CARBONIC ANHYDRASE 1; Carbonic anhydrase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGATTGTACTATTGCCCAAACTCTACAG |
| Internal bar code: | AAGCGACAATGATCGGAGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 89.9135 |
| LEAP-Seq n confirming: | 2808 |
| LEAP-Seq n nonconfirming: | 315 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGTCATGCCAGTAGTGT |
| Suggested primer 2: | TAGCTTTGCGCCTAGATGGT |