Insertion junction: LMJ.RY0402.196389_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.196389
Chromosome:chromosome 2
Location:2105754
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g089200 HLM4,SET3 (1 of 1) PTHR22884//PTHR22884:SF370 - SET DOMAIN PROTEINS // HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-9 SPECIFIC SUVH5-RELATED; Histone-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACGCCAGACTCACACGCCCTCACA
Internal bar code:ACGTAACGCAGGATGTACGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:632
LEAP-Seq percent confirming:99.5436
LEAP-Seq n confirming:1745
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TTAACAACCGTTCGCATGAA
Suggested primer 2:CTTGCTCTCCGTCATGAACA