| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196454 |
| Chromosome: | chromosome 1 |
| Location: | 6917639 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049826 | PHC59 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGACTCCTCGGAATGCATGTACCGTAC |
| Internal bar code: | AGAGGGGATAGAGAAACGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 243 |
| LEAP-Seq percent confirming: | 94.0 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTTTGACTGGAGCTGCG |
| Suggested primer 2: | CGTCTGAGTAGGCGATGTCA |