| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196462 |
| Chromosome: | chromosome 2 |
| Location: | 2448816 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091700 | RIB72 | Flagellar protofilament ribbon protein 72; (1 of 1) PF06565//PF13499 - Repeat of unknown function (DUF1126) (DUF1126) // EF-hand domain pair (EF-hand_7) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACAAGCCCATGCACAACTTTTCGGACA |
| Internal bar code: | GGAGCCCCCCGACCCCGTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 87.0634 |
| LEAP-Seq n confirming: | 673 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGAGGGTGTCGTTCATT |
| Suggested primer 2: | CTGTAAGCAGGGAGAGGCAC |