| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196464 |
| Chromosome: | chromosome 16 |
| Location: | 4117918 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669350 | (1 of 37) PF13639 - Ring finger domain (zf-RING_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCGGCGATGGGTCGGTGGACATCTCTG |
| Internal bar code: | GCGGAGCAAATGCAGAAGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 99.5109 |
| LEAP-Seq n confirming: | 2645 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTTTACTCCAACCTGC |
| Suggested primer 2: | TGAACACGATGAGCAGAAGC |