| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196470 |
| Chromosome: | chromosome_10 |
| Location: | 5450145 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g458600 | FAP245 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGTCGCCTGGGCTGAAGCCGTTTCTCCCA |
| Internal bar code: | TCAAACGGCTTCGCTGTATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 380 |
| LEAP-Seq percent confirming: | 99.5547 |
| LEAP-Seq n confirming: | 2459 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCTTCTGGTTGGCTTC |
| Suggested primer 2: | GGAGGAATGACCTCGCATAG |