Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.196477 |
Chromosome: | chromosome 6 |
Location: | 6474799 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g292950 | (1 of 1) K03505 - DNA polymerase delta subunit 4 (POLD4) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACACGAAACGTGGCATGCCCCCCAGTC |
Internal bar code: | CGGGCTTGTGACGGGCTGATCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 982 |
LEAP-Seq percent confirming: | 98.7483 |
LEAP-Seq n confirming: | 3629 |
LEAP-Seq n nonconfirming: | 46 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCGGTTAGGCCAGTCTCAG |
Suggested primer 2: | AGGTATTGGTGTTTGCGGAG |