| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196503 |
| Chromosome: | chromosome 8 |
| Location: | 1726248 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g366050 | (1 of 13) IPR000086//IPR015797 - NUDIX hydrolase domain // NUDIX hydrolase domain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGCCACGCCACGCGGCGCTGGACTCCTG |
| Internal bar code: | TGGGGACAGCGGGCAGAAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 90.493 |
| LEAP-Seq n confirming: | 257 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACTGGTCGTTGGTTGGA |
| Suggested primer 2: | ATCCCTACCCAGCCATCTCT |