Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.196536 |
Chromosome: | chromosome_2 |
Location: | 5088121 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g104900 | FAP204 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTCCAGCGGAAAGAAGTAAAAGGAGCAGCA |
Internal bar code: | CCAGAGTCCGAAACGCTGGTCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 828 |
LEAP-Seq percent confirming: | 99.6488 |
LEAP-Seq n confirming: | 2270 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATTGGTTGGCTTTGTTTGC |
Suggested primer 2: | CTGTACCGAGACATCGCTGA |