| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196538 |
| Chromosome: | chromosome 7 |
| Location: | 155757 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313206 | (1 of 2) 2.7.7.48 - RNA-directed RNA polymerase / RNA nucleotidyltransferase (RNA-directed) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAGGGATGGAAGTGGGGAGGAAGGTGG |
| Internal bar code: | TGGACCTCGGGGACACCAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 62.7273 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGGATGTTCGTGTG |
| Suggested primer 2: | AAGGTAGGTGCGTGTGAGGT |