| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196561 |
| Chromosome: | chromosome 2 |
| Location: | 5524540 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108450 | FAP280 | Flagellar Associated Protein 280, transcriptional coactivator-like; (1 of 1) K03627 - putative transcription factor (MBF1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTTGATGGGCTGGGGCTGGACCGTGG |
| Internal bar code: | AGGAGTTTCCAAGACGCGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1006 |
| LEAP-Seq percent confirming: | 98.1838 |
| LEAP-Seq n confirming: | 919 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTAACAAAACAGCGCCT |
| Suggested primer 2: | ACAAAGCATGGCTTACCCAC |