| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196565 |
| Chromosome: | chromosome 3 |
| Location: | 5155430 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182150 | TEF8 | (1 of 1) PTHR30373:SF2 - UPF0603 PROTEIN YGCG; Thylakoid Lumen Protein 18.3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATCGCGAACAGTCGTACATGTCTTGGTT |
| Internal bar code: | TCTGCAGCCAGGCCCTCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 99.834 |
| LEAP-Seq n confirming: | 1203 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCGATCTCAGGTACAGTG |
| Suggested primer 2: | TTTTTGCTGCAATGAACAGC |