| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196595 |
| Chromosome: | chromosome_6 |
| Location: | 4067881 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278212 | CGL46 | Predicted protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCCGTCACCCATGACATAGGAAAGCAGAC |
| Internal bar code: | TTCGAGGACTGGAGTTGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGGCTGACTCATTGCTG |
| Suggested primer 2: | TCGGCTAAGCTTTCAATCGT |