| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196597 |
| Chromosome: | chromosome 6 |
| Location: | 1459576 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259450 | (1 of 24) IPR006311 - Twin-arginine translocation pathway, signal sequence | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCCTCACACATGTCACCCACCCTCATC |
| Internal bar code: | TGGATAGGACACATCTCTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 123 |
| LEAP-Seq percent confirming: | 84.2857 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGCACTACTCGCAAGGT |
| Suggested primer 2: | TGACTATCTCACGCGTCGTC |