| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196607 |
| Chromosome: | chromosome 17 |
| Location: | 2246241 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713600 | SUB13 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATATGCATGCCAAGGTAGAACTTAAAC |
| Internal bar code: | TCCGGAAGGGACGCGTAGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 99.7704 |
| LEAP-Seq n confirming: | 5215 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACGG |
| Suggested primer 2: | GCCTGCTCGTCATGTGTTTA |