| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196613 |
| Chromosome: | chromosome 8 |
| Location: | 4451228 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382300 | CCB4 | CGLD23 protein, required for cyt b6 assembly | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCACGGCCACCCCAGGCGACCAGACCGG |
| Internal bar code: | AGATCGGGAGGAGATCGGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.7547 |
| LEAP-Seq n confirming: | 4880 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGTAGAGGAGCACCAGC |
| Suggested primer 2: | CTTCGTGCCGGAAGTAAGAG |