| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196617 |
| Chromosome: | chromosome 6 |
| Location: | 855066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g254650 | (1 of 1) K13127 - RING finger protein 113A (RNF113A, CWC24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACCGCAGCACCTATGCAAGAGGGGGCG |
| Internal bar code: | GGGAGCGTGTTTGGACCCGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 96.7298 |
| LEAP-Seq n confirming: | 1124 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAAGGGCATGAACGCATA |
| Suggested primer 2: | TCGTCTACACAGCTCATCCG |