| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196618 |
| Chromosome: | chromosome 6 |
| Location: | 5489715 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284750 | EFG3 | (1 of 1) K03267 - peptide chain release factor subunit 3 (ERF3, GSPT); Translation elongation factor Tu family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAGTGATGATGGCAGGATGCTGATGCA |
| Internal bar code: | AAGAGACTCGTGAGCATTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 99.6543 |
| LEAP-Seq n confirming: | 4900 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTACCACTCACCATCCGC |
| Suggested primer 2: | CGACTTGACGAAATTGAGCA |