Insertion junction: LMJ.RY0402.196623_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.196623
Chromosome:chromosome 6
Location:6661765
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g294100 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAGAATGCCATGCGCGAGCTGGTGAAGG
Internal bar code:TGACCTACAAAGACACCGTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1108
LEAP-Seq percent confirming:89.6977
LEAP-Seq n confirming:6112
LEAP-Seq n nonconfirming:702
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCAAAGAGAAGCCCAAGA
Suggested primer 2:CTGCGTCTCAACTTTCTCCC