| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196629 |
| Chromosome: | chromosome 16 |
| Location: | 7760858 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692228 | MARS1 | Mutant Affecting Retrograde Signaling 1; (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCAGCTACTGTGGACTGTGGAAATCCCG |
| Internal bar code: | AGGTTCCCGCTGCTAGCTGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 98.4772 |
| LEAP-Seq n confirming: | 194 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCATTGAAGACACAAGGT |
| Suggested primer 2: | CCAAGCTTTAGGGTGGCATA |