| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196630 |
| Chromosome: | chromosome_4 |
| Location: | 2931092 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g224650 | FAP296 | Flagellar Associated importin alpha-like protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCGGCCCTGCGAAAACCGCGCGCGCGGG |
| Internal bar code: | GAGCGACTCACTACCGCCACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 99.6844 |
| LEAP-Seq n confirming: | 3474 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGGTATGGGACAGAGCG |
| Suggested primer 2: | CTGGACAGGCCGTAAGTAGC |