| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196645 |
| Chromosome: | chromosome 10 |
| Location: | 425552 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420650 | (1 of 24) IPR001680//IPR017986//IPR020472 - WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTTGGCCAGCTGCAGACCAATGAGCTC |
| Internal bar code: | AGGGGGATCATTTCGTATGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 99.0805 |
| LEAP-Seq n confirming: | 862 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGAGGGTAGTTGAGGGC |
| Suggested primer 2: | CCTCCTACCCTCCTACCCTG |