| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196686 |
| Chromosome: | chromosome 12 |
| Location: | 2114446 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509100 | FAP307 | Flagellar Associated Protein 307 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAGCACAGCGCTGCAAGCCACGCTGTA |
| Internal bar code: | GCGTTTTACGGTTGTACGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 390 |
| LEAP-Seq percent confirming: | 99.4387 |
| LEAP-Seq n confirming: | 1240 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACACTATCTCAGTGCGGA |
| Suggested primer 2: | GACTTTTTCGGCTGCTGTTC |