| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196694 |
| Chromosome: | chromosome 12 |
| Location: | 9607631 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550277 | MRS2 | Mg2+ transporter protein, CorA-like and putative mitochondrial splicing factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTTGCTTGCGGTGGGTGAAACTCTGCG |
| Internal bar code: | TGCTTTTGCTGTGCCCACGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 614 |
| LEAP-Seq percent confirming: | 99.6651 |
| LEAP-Seq n confirming: | 7738 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGTCAGCGATGTATGGTA |
| Suggested primer 2: | TGCCCTCCTTGTTTCTTACG |