| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196701 |
| Chromosome: | chromosome 12 |
| Location: | 1292451 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489050 | PCT2,CDS2 | Phosphatidate cytidylyltransferase; (1 of 1) PTHR13773:SF3 - PHOSPHATIDATE CYTIDYLYLTRANSFERASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGCTGTCCAGCAATCGGGCAAGTGTT |
| Internal bar code: | AATATATTTCGATTGTCCCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 99.7592 |
| LEAP-Seq n confirming: | 4557 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGCATGCCCCTGTTAG |
| Suggested primer 2: | AAGGTGACAGGGAACAAACG |