| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196702 |
| Chromosome: | chromosome 3 |
| Location: | 5557238 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186450 | LNM1 | Lysine N-methylase; (1 of 5) PTHR13271//PTHR13271:SF28 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGCAATGCAGCTAACCTGGACCTCTT |
| Internal bar code: | GATGAACGTTCTTTAATCTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 99.4431 |
| LEAP-Seq n confirming: | 2500 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTAACTGGATTGGCCTG |
| Suggested primer 2: | GGACATCCGCAATCAGAAGT |