| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196707 |
| Chromosome: | chromosome 3 |
| Location: | 6177566 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192250 | PHC69 | (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 69 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGCTGGTATTTACAAACCCACCTCTGT |
| Internal bar code: | GATATCCTTTTAATGTAGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 99.4784 |
| LEAP-Seq n confirming: | 3242 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTGCCGTGTTAATGGTG |
| Suggested primer 2: | GTCGCGTTCAAACTGAACCT |