| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196729 |
| Chromosome: | chromosome 2 |
| Location: | 2457102 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091850 | (1 of 1) 4.2.1.18 - Methylglutaconyl-CoA hydratase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGGTGGTGTGGGGGGGGCAGGGCCGCT |
| Internal bar code: | TAAAGAGCGTGTGCGTAGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 99.434 |
| LEAP-Seq n confirming: | 5270 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGGGATATCCTGGCAGA |
| Suggested primer 2: | GACTCGTTTCCGGATGTTGT |