| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196737 |
| Chromosome: | chromosome 7 |
| Location: | 6328997 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357157 | (1 of 1) 1.14.13.99 - 24-hydroxycholesterol 7-alpha-hydroxylase / 24-hydroxycholesterol 7-alpha-monooxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGGGGTAAGCAATCCCTTCCCACCTTC |
| Internal bar code: | TTCAGTTCGGGGGGGCTTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 81.6254 |
| LEAP-Seq n confirming: | 231 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | ACTCAGTATAACCCGTGCCG |