| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196750 |
| Chromosome: | chromosome 2 |
| Location: | 1053628 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080700 | BIP1 | (1 of 2) K09490 - heat shock 70kDa protein 5 (HSPA5, BIP); Endoplasmic reticulum associated Hsp70 protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGTTTCGCACGCATACACATCAAGCAC |
| Internal bar code: | GAGTACCGATGGCGGAGTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 99.3406 |
| LEAP-Seq n confirming: | 3013 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCCTAGAGGCTTGTGTG |
| Suggested primer 2: | CAAGCTGAAGGAGGTTGAGG |