Insertion junction: LMJ.RY0402.196758_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.196758
Chromosome:chromosome 12
Location:7883933
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g552750 (1 of 2) PF04520 - Senescence regulator (Senescence_reg) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGACCCAGACACTTCATCGGCCAAGTCT
Internal bar code:CCGTCGCGGAAGTGGTGCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:929
LEAP-Seq percent confirming:99.6321
LEAP-Seq n confirming:3791
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGTGGCCAACCATACTTT
Suggested primer 2:TATGCATCTCTTGCACCAGC