Insertion junction: LMJ.RY0402.196800_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.196800
Chromosome:chromosome 13
Location:4368173
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g602901 CNX2 (1 of 1) PTHR22960:SF0 - MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1; Cofactor of nitrate reductase and xanthine dehydrogenase 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCCGTCCGCCAGCAGTCGCAGTCGGTT
Internal bar code:CCACGAGCGAAATTGCCACGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:605
LEAP-Seq percent confirming:99.6743
LEAP-Seq n confirming:306
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTACCCGCCAATTGTGAT
Suggested primer 2:GCTTTGATGCCGAGCTAAAC