Insertion junction: LMJ.RY0402.196835_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.196835
Chromosome:chromosome 6
Location:2800205
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g271188 (1 of 12) IPR004843//IPR029052 - Calcineurin-like phosphoesterase domain, apaH type // Metallo-dependent phosphatase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGTCCGGGGCCTGTGCTCCTATGGTGTG
Internal bar code:CGGACTAGCTCAACCCATGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:401
LEAP-Seq percent confirming:98.8166
LEAP-Seq n confirming:668
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTCGGTCAGGAACAACTA
Suggested primer 2:AGCTCCTGGCTTCACACAGT