| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196856 |
| Chromosome: | chromosome 3 |
| Location: | 6263727 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192850 | (1 of 1) 2.3.1.193 - tRNA(Met) cytidine acetyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTCCCGCGTATTGAGTCAGCGTGGTTCC |
| Internal bar code: | GGCAGTTTGGGTCCCTAGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 220 |
| LEAP-Seq percent confirming: | 99.3135 |
| LEAP-Seq n confirming: | 3038 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATACCAAGACTCGCCCAC |
| Suggested primer 2: | CGTGGTGATCCTTTTCTGGT |