| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196875 |
| Chromosome: | chromosome 6 |
| Location: | 7419330 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299800 | LCL1 | Long-chain-fatty-acid-CoA ligase; (1 of 1) 6.2.1.20 - Long-chain-fatty-acid--[acyl-carrier-protein] ligase / Acyl-[acyl-carrier-protein] synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGCGATGTGCGCCACGTGGTCAAAGGG |
| Internal bar code: | CCCTTCGGTGGTCCCCACCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 93.9536 |
| LEAP-Seq n confirming: | 12058 |
| LEAP-Seq n nonconfirming: | 776 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATACCGTACTCCTTCCA |
| Suggested primer 2: | AAAGCGCTACTGAAGACCGA |