| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196877 |
| Chromosome: | chromosome 8 |
| Location: | 3630431 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376200 | (1 of 1) IPR000104//IPR013026//IPR019734 - Antifreeze protein, type I // Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTAGGAAATGCCCCCGAGCTGCGGCCCC |
| Internal bar code: | CCAGGCGAGTTCCGGTTTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 745 |
| LEAP-Seq percent confirming: | 99.4615 |
| LEAP-Seq n confirming: | 8496 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACATGCCTTTCAACCGTTC |
| Suggested primer 2: | GATGTCTTGGGTCAGACGGT |