| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.196887 |
| Chromosome: | chromosome_14 |
| Location: | 2922612 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g627576 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACGTGCTTGCGCTCCCGTAGGTCCTCCAG |
| Internal bar code: | GAACGTATACGCAACGTTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 804 |
| LEAP-Seq percent confirming: | 99.5629 |
| LEAP-Seq n confirming: | 1139 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACTCTCCACCCAC |
| Suggested primer 2: | GGTTCGTAAGTACCAGCCCA |