| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196920 |
| Chromosome: | chromosome 10 |
| Location: | 1092126 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425450 | AOF5 | (1 of 1) 1.5.99.4 - Nicotine dehydrogenase; Flavin-containing amine oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTACGAACAAGGCAGATAAGGACGAGCTA |
| Internal bar code: | CTTGGATTCTGCACGTGTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 252 |
| LEAP-Seq percent confirming: | 99.6833 |
| LEAP-Seq n confirming: | 4407 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTAGCAGCGACTTCTCAA |
| Suggested primer 2: | GCAAGAGAGAGGTTATGGCG |