Insertion junction: LMJ.RY0402.196944_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.196944
Chromosome:chromosome 2
Location:6848385
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g119450 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGTGTCCCCACCCCTGCCGCCCCCTCCC
Internal bar code:AGGAGGGCGAGAGAGCCGTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:145
LEAP-Seq percent confirming:93.4837
LEAP-Seq n confirming:373
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGAGTACAACGTGGGCTT
Suggested primer 2:AGACGTAGACTGCGGAGGAA