| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.196946 |
| Chromosome: | chromosome 1 |
| Location: | 3363008 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021251 | ASL,ARG7 | Argininosuccinate lyase; (1 of 1) 4.3.2.1 - Argininosuccinate lyase / Omega-N-(L-arginino)succinate arginine-lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGGGAGTGGGCGGGGAGTGGAAGAGTC |
| Internal bar code: | GTCGAGCATTCCAGCGTTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 99.5037 |
| LEAP-Seq n confirming: | 2005 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCAGAAACCAGAAGCAC |
| Suggested primer 2: | CACCTTACTGTGGCCATGTG |