| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196961 |
| Chromosome: | chromosome 9 |
| Location: | 3067267 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387150 | HSF1 | (1 of 1) PTHR10015:SF162 - HEAT STRESS TRANSCRIPTION FACTOR A-1A-RELATED; Heat shock transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGAGGGGACAGGCAAGGCTGGCAAGAC |
| Internal bar code: | GTGGCGGTAGATCTTATGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 248 |
| LEAP-Seq percent confirming: | 98.9933 |
| LEAP-Seq n confirming: | 295 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCCCGGGTTGGTATGTGT |
| Suggested primer 2: | TGATATGTGCTGCCTTAGCG |