| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.196970 |
| Chromosome: | chromosome_6 |
| Location: | 2166339 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g265950 | DHC3 | Flagellar inner arm dynein heavy chain, novel minor type dynein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTCCGGTTGTGGCTGACCAGCATGCCCAC |
| Internal bar code: | TAGGCGATCTCTAGAAGTCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 53.7487 |
| LEAP-Seq n confirming: | 2545 |
| LEAP-Seq n nonconfirming: | 2190 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGTTCACCGACTCCTC |
| Suggested primer 2: | ACCCTCCACCAGGAACATCT |