| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.196972 |
| Chromosome: | chromosome 14 |
| Location: | 2221628 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g622901 | (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGTACGGCTTTTCCAACCACCTTTTAC |
| Internal bar code: | TGAACGTTTTGGCCGTCGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 204 |
| LEAP-Seq percent confirming: | 99.3865 |
| LEAP-Seq n confirming: | 324 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCGATGGTCTAGTCTAC |
| Suggested primer 2: | GCGTCATCAGCAAGTGTGTT |