| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197022 |
| Chromosome: | chromosome 3 |
| Location: | 5142190 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182050 | LCS1 | (1 of 4) K01897 - long-chain acyl-CoA synthetase (ACSL, fadD); Long-chain acyl-CoA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTGACCGCATGAGCGGAGGGGTGCCGC |
| Internal bar code: | CGGTGGACAGGTCACACAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 903 |
| LEAP-Seq percent confirming: | 98.5145 |
| LEAP-Seq n confirming: | 1459 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAACCCTGCAATCTGTT |
| Suggested primer 2: | ATATTGCGGTGGAGAAGGTG |