| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.197023 |
| Chromosome: | chromosome 12 |
| Location: | 1152648 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g490150 | HEL48 | (1 of 1) K03654 - ATP-dependent DNA helicase RecQ (recQ); DEAD/DEAH box helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACCTATCCGTCCACCGCCCCCCTCCGG |
| Internal bar code: | GTAGGATCACCGTGCTTAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTACCTACGATGTGGCG |
| Suggested primer 2: | AGCCACACACAATGCTTCAG |