Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.197026 |
Chromosome: | chromosome 8 |
Location: | 4980081 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g385800 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCGCCGTCCTCGTTGCCGCCGCCGTGT |
Internal bar code: | CGGTGCTGTACTCCGACGAGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 540 |
LEAP-Seq percent confirming: | 95.6522 |
LEAP-Seq n confirming: | 66 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACCGGATGAAGAGCCAGTC |
Suggested primer 2: | GTTTTGGTTTTCAGCGGGTA |