| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.197042 |
| Chromosome: | chromosome 9 |
| Location: | 5500690 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400441 | (1 of 1) K11323 - histone arginine demethylase JMJD6 [EC:1.14.11.-] (JMJD6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGCCGCTATCTCGCGGTCCGTCAGCAC |
| Internal bar code: | TTGAGTAGCCGCTTGCCAAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 99.6328 |
| LEAP-Seq n confirming: | 5698 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTGCAACATTTGGGTTG |
| Suggested primer 2: | GATACATTGATGCGGTGTGC |